Effect of duration of mycelia fragmentation and concentration on virulence of Mycosphaerella fijiensis in banana
Keywords:banana, Mycosphaerella genus, severity, area under disease progress curve
Mycelia from 3 isolates of Mycosphaerella fijiensis (Mak 01, Mak 02 and Kaw 10) were fragmented for 3 and 1 minutes, and tested at 5 mg mL-1, 10 mg mL-1 and 15 mg mL-1 for virulence in the screen house. Disease severity was found to depend on the level of fragmentation and concentration. Inoculation of Gros Michel plantlets with mycelia fragmented for 3 minutes resulted in significantly higher levels of disease severity (AUDPC) than fragmentation for 1 minute for all the isolates. For both times of fragmentation, AUDPC increased with concentration. The highest and lowest AUDPCs for both times of fragmentation were achieved with concentrations of 15 and 5 mg mL-1, respectively. Overall, all the three isolates demonstrated high levels of virulence, with Mak 01 causing the highest severity (AUDPC - 98.5) when compared to Mak 02 (AUDPC - 93.5) and Kaw 10 (AUDPC - 92.2) for 3-minute fragmentation and concentration of 15 mg mL-1. Collectively, our data demonstrate that a potent inoculum of Mycosphaerella fijiensis for in vitro studies can be reliably prepared by fragmenting weighed mycelia in a blender.
Balint-Kurti, P., & Churchill, A. C. L. 2004. Towards a molecular understanding of Mycosphaerella/banana interactions. In: Jain, S. M., & Swennen, R. editors. Banana Improvement: Cellular, Molecular Biology, and Induced Mutations. Plymouth: Science Publishers, Inc. pp. 147-159.
Burt, P. J. A., Rosenberg, L. J., Rutter, J., Ramirez, F., & Gonzales, H. (1999). Forecasting the airborne spread of Mycosphaerella fijiensis, a cause of Black Sigatoka disease on banana: estimations of number of perithecia and ascospores. Annals of Applied Biology, 135(1), 369-377. https://doi.org/10.1111/j.1744-7348.1999.tb00863.x DOI: https://doi.org/10.1111/j.1744-7348.1999.tb00863.x
Churchill, A. C. L. (2011). Mycosphaerella fijiensis, the black leaf streak pathogen of banana: progress towards understanding pathogen biology and detection, disease development, and the challenges of control. Molecular Plant
Donzelli, B. G. G., & Churchill, A. C. L. (2007). A quantitative assay using mycelial fragments to assess virulence of Mycosphaerella fijiensis. Phytopathology, 97(8), 916-929. https://doi.org/10.1094/PHYTO-97-8-0916 DOI: https://doi.org/10.1094/PHYTO-97-8-0916
Fullerton, R. A., & Olsen, T. L. (1995). Pathogenic variability in Mycosphaerella fijiensis Morelet, the cause of Black Sigatoka in banana and plantain. New Zealand Journal of Crop and Horticultural Science, 23(1), 39-48. https://doi.org/10.1080/01140671.1995.9513866 DOI: https://doi.org/10.1080/01140671.1995.9513866
Kumakech, A., Lyngs Jørgensen, H. J., Edema, R., & Okori, P. (2015). Efficient screening procedure for black sigatoka disease of banana. African Crop Science Journal, 23(4), 387-397. https://doi.org/10.4314/acsj.v23i4.8 DOI: https://doi.org/10.4314/acsj.v23i4.8
Madden, L. V., Hughes, G., & Vanden Bosch, F. (2007). The study of plant disease epidemics. American Phytopathological Society. St. Paul Minnesota, USA. https://apsjournals.apsnet.org/doi/pdf/10.1094/9780890545058.fm
Marin, D. H., Romero, R. A., Guzmán, M., & Sutton, T. B. (2003). Black Sigatoka: An increasing threat to banana cultivation. Plant Disease, 87(3), 208-222. https://doi.org/10.1094/PDIS.2003.87.3.208 DOI: https://doi.org/10.1094/PDIS.2003.87.3.208
Noar, R. D., & Daub, M. E. (2016). Bioinformatics prediction of polyketide synthase gene clusters from Mycosphaerella fijiensis. PLoS ONE, 11, 1-31. https://doi.org/10.1371/journal.pone.0158471 DOI: https://doi.org/10.1371/journal.pone.0158471
Stover, R. H. (1976). Distribution and cultural characteristics of the pathogens causing banana leaf spot. Tropical Agriculture (Trinidad), 53(2), 111-114. https://www.cabdirect.org/cabdirect/abstract/19761326729
Tushemereirwe, W. K., Kangire, A., Kubiriba, J., Nakyanjzi, M., & Gold, S. (2004). Field reaction of banana cultivars to Black Sigatoka in Uganda. African Crop Science Journal, 12, 19-26. DOI: https://doi.org/10.4314/acsj.v12i1.27658
How to Cite
Copyright (c) 2023 Alfred Kumakech, Richard Edema, Patrick Okori
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.